旋毛虫成虫排泄分泌抗原检测唾液中抗旋毛虫IgG抗体的研究

Research on the detection of IgG antibodies in saliva with Trichinella spiralis adult worm excretory-secretory antigen

目的评价旋毛虫（Trichinella spiralis）成虫排泄分泌抗原（adult worm excretory—secretory antigen，AWESA）作为诊断抗原检测旋毛虫感染日本大耳兔唾液中抗旋毛虫IgG抗体的可行性。方法建立旋毛虫感染日本大耳兔和对照组兔动物模型，采集感染前和感染后1～6周兔唾液和血清以及对照组兔唾液和血清。制备AWESA，建立AWESA作为诊断抗原的间接酶联免疫吸附试验（enzyme—linked immunosorbent assay，ELISA），以市售旋毛虫IgG抗体检测试剂盒作为对照，测定感染前和感染后1～6周兔唾液和血清以及对照组兔唾液和血清中抗旋毛虫IgG抗体。AWESA和试剂盒测得的唾液A值和血清4值进行线性相关分析，AWESA和试剂盒测得的唾液阳性率和血清阳性率分别进行，检验。结果AWESA检测唾液和血清特异性IgG抗体阳性率依次为0、5％、20％、40％、60％、85％、90％，0、30％、60％、85％、95％、100％、100％，除感染后0、1、2周外其余各周唾液A值与血清A值呈显著线性相关（P〉0．05、P〉0．05、P〉0．05、P〈0．05、P〈0．05、P〈0．05、P〈0．05）。市售旋毛虫IgG抗体检测试剂盒检测旋毛虫感染前和感染后兔唾液和血清中特异性IgG抗体阳性率依次为0、15％、20％、40％、55％、75％、90％，0、35％、60％、95％、95％、100％、100％，除0、1、3周外其余各周唾液A值与血清A值呈线性相关（P〉0．05、P〉0．05、P〈0．05、P〉0．05、P〈0．05、P〈0．05、P〈0．05）。结论AWESA与市售试剂盒检测唾液和血清中抗旋毛虫IgG抗体的阳性率具有一致性。

Objective To evaluate＇the feasibility of the detection of IgG antibodies in saliva with Trichinella spiralis adult worm excretory-secretory antigen （AWESA）. Methods Animal model of Japanese big ears rabbits infected with T. spiralis was established, AWESA was prepared. Saliva and serum of the rabbits before and 1-6 weeks post infection and that of the control group were collected. Taking commercial kit detecting anti-T, spiralls IgG antibody as control, indirect enzyme-linked immunosorbent assay （ ELISA ） using AWESA was developed. Anti-T. spiralis IgG antibodies in saliva and serum of the rabbits before and 1-6 weeks post infection and that of the control group were detected with ELISA. A values of saliva and serum from the detection with AWESA and commercial kit were tested with linear regression analysis. The comparisons of positive rates from the detection with AWESA and commercial kit were determined by the chi-squarc test. Results The positive rates of saliva and serum from rabbits infected with T. spiralis before infection and 1-6 weeks postinfection obtained by AWESA were 0,5% , 20% , 40% , 60%, 85% , 90% and 0,30% , 60% , 85% , 95% , 100% , 100% , respectively. There were significant linear correlations between A values of saliva and A values of serum every week, except for before and 1 week and 2 week post-infection（ P 〉 0.05, P 〉 0.05,P 〉 0.05, P 〈 0.05, P 〈 0.05, P 〈 0.05, P 〈 0.05, respectively）. The positive rates of saliva and serum from rabbits infected with 7：. spirali. before and 1-6 weeks post-Infection obtained by commercial kit detecting anti-T, spiralis IgG antibody were 0,15% ,20% ,40% ,55% ,75% ,90% and 0,35% ,60% ,95% ,95% , 100% , 100% , respectively. There were significant correlations between A values of saliva and A values of serum every week, except that of before infection, 1 week and 3 week post-infection （ P 〉 0.05, P 〉 0.05, P 〈 0.05, P 〉 0.05, P 〈 0.05, P 〈 0.05 ,P 〈 0.05, respectively）. Conclusion The positive rates of the detection of anti-T, spiralis IgG antibodies in saliva and serum by AWESA were of consistency with that by commercial kit.