盐胁迫对菠菜幼苗抗性生理的研究

Physiological Effects of Different Concentration of Na Ion Stress on Spinacia oleracea Seedling

本文采用砂培法,研究了不同浓度的盐胁迫对菠菜幼苗叶绿素、丙二醛和脯氨酸含量以及SOD、POD、CAT等抗氧化酶活性的影响。结果表明:Na+低浓度(10、20 mg/L)胁迫时,叶绿素含量和抗氧化酶活性都高于对照组;而在高浓度(>40 mg/L)条件下,酶的活性下降、叶绿素含量降低;随Na+胁迫浓度的增大,丙二醛含量呈上升趋势,表明脂质过氧化程度在增加;脯氨酸含量表现出持续增加,增强植株对盐的抗性。

The different concentrations of Na^＋ making stress on Spinacia oleracea Linn of chlorophyll , MDA and proline and several antioxidant enzymes activity were studied by using the method of indoor sand - culture. The Results show that, When Na^＋ solution is in low concentration （10, 20 mg/L）, the chlorophyll content, SOD, POD and the CAT enzyme activity all are higher than the control group; When under high potency （ 〉 40mg/L） condition, enzyme system is out of balance due to the increasing oxygen, plant of resistance capability is lower, several enzymes and chlorophyll show a trend of decline; In the process of experiment, proline shows a increasing trend, improving resistance of the plant . MDA is also rising, and MDA is usually used for the measurement of lipid peroxidation.