碳点共振光散射法测定溶菌酶

Carbon Dots Used for the Detection of Lysozyme with Resonance Light Scattering Signals

利用共振光散射(RLS)技术研究了碳点与溶菌酶的相互作用.带负电荷的碳点与带正电荷的溶菌酶通过静电引力相结合,形成大颗粒复合物,具有很强的光散射信号,并且信号不易受酸度和离子强度影响.增强的RLS强度与溶菌酶浓度在71.5μg/L～14.3mg/L范围内呈线性关系,检出限为37.76μg/L(3σ).此方法应用于溶菌酶含片中溶菌酶含量的检测,回收率在95.8%～98.9%之间,相对标准偏差小于4.4%.

The interaction between carbon dots and lysozyme was studied with resonance light scattering technique.It was found that the negatively charged carbon dots could bind with positively charged lysozyme through electrostatic attraction,forming a large complex that has strong light scattering signals and is unlikely to be influenced by pH and ionic strength.The enhanced RLS intensity was found to be linearly proportional to the concentration of lysozyme in the ranges of 71.5 μg/L-14.3 mg/L.Under the optimum conditions,the detection limit of lysozyme of 37.76 μg/L（3σ） could be available.The method was applied to the detection of the lysozyme in lyszome buccal tablets samples with the recovery from 95.8% to 98.9% and the RSD lower than 4.4%.