钯(Ⅱ)-托拉塞米反应体系的共振瑞利散射和共振非线性散射光谱及其分析应用

Resonance Rayleigh Scattering and Resonance Nonlinear Scattering Spectra of Pd(Ⅱ)-Torasemide System and Their Analytical Application

在pH=4.0～7.0的Britton-Robinson(BR)缓冲溶液中,托拉塞米与Pd(Ⅱ)形成1∶1的螯合物,此时将引起共振瑞利散射(RRS)、二级散射(SOS)和倍频散射(FDS)显著增强,其最大RRS,SOS和FDS波长分别位于340,586,349 nm附近.在一定范围内,3种散射增强与托拉塞米的浓度成正比,方法具有较高的灵敏度,对托拉塞米的检出限(3σ)在1.1～5.5 ng/mL之间.考察了适宜的反应条件及共存物质的影响,表明方法有良好的选择性.据此,提出了测定痕量托拉塞米的灵敏、简便和快速的新方法.

In a Britton-Robinson buffer solution of pH=4.0-7.0,torasemide reacts with Pd（Ⅱ） to form a 1∶1 chelate,which results in great enhancement of the intensities of resonance Rayleigh scattering（RRS）,second-order scattering（SOS） and frequency doubling scattering（FDS）.The maximum RRS,SOS,FDS wavelength are located at 340,586,349 nm,respectively.The enhanced RRS,SOS and FDS intensities are directly proportional to the concentration of torasemide in a certain range.The methods have high sensitivities and the detection limits for TOR are between 1.1 and 5.5 ng/mL.The optimum conditions and the effects of coexisting substances are investigated and the results show that the methods exhibit a good selectivity.Based on the above researches,a highly sensitive,simple and quick method for the determination of torasemide has been developed.