人工培殖冬虫夏草多糖的含量测定

Determination of Polysaccharides in Cultivated Cordyceps sinensis

目的:为人工栽培冬虫夏草的药用提供依据.方法:用蒽酮-硫酸法测定野生冬虫夏草与人工培殖冬虫夏草多糖含量.结果:葡萄糖质量浓度在0.02～0.12 mg/mL时,与吸光值(OD620值)有良好线性关系,线性方程Y=0.185 4x-0.000 4,相关系数r=0.999 7.人工培殖冬虫夏草和野生冬虫夏草多糖含量分别为5.83%(2008年)、4.18%(2007年)、2.83%(2006年)、4.79%(2005年)和4.16%(野生).结论:人工培殖冬虫夏草与野生冬虫夏草的多糖含量基本一致.该方法精密度高、重现性好和回收率达94%～96%.

Objective： To provide a basis for the assessment of medicinal value of cultivated Cordyceps sinensis.Methods： The polysaccharide contents of wild and cultivated C.sinensis were determined with the anthrone sulphuric acid method.Results： In the range of 0.02-0.12 mg/mL,glucose concentration showed a good linearity with absorbance values（OD620 value）（r=0.999 7）,the linear equation being Y=0.185 4x-0.000 4.The contents of polysaccharides were 5.83%（2008）,4.18%（2007）,2.83%（2006） and 4.79%（2005） for the cultivated type and 4.16%（2008） for the wild type.Conclusion： Polysaccharide contents of cultivated C.sinensis are similar to those of the wild type.The anthrone-sulfuric acid method has good sensitivity and reproducibility,with a recovery rate of 94%-96%.