肠易激综合征患者结肠黏膜中MC与VIP的表达

The expression of vasoactive intestinal peptide and mast cells in colonic mucosa of patiens with irritable bowel syndrome

目的:探讨肥大细胞(MC)与血管活性肠肽(VIP)在肠易激综合征(IBS)患者结肠黏膜中的表达情况及二者的联系,为神经-内分泌-免疫网络分析IBS的发病机制提供一定理论依据。方法:选取腹泻型和便秘型IBS患者及正常对照者各20例,对其结肠黏膜活检标本进行免疫组化SABC法和甲苯胺蓝法染色后,对比观察MC和VIP表达情况。结果:IBS患者回盲部和乙状结肠部MC数和VIP免疫阳性神经纤维数均较对照组显著增多(P<0.01),而且IBS患者回盲部MC数和VIP免疫阳性神经纤维均较乙状结肠部MC数和VIP免疫阳性神经纤维明显增多。结论:MC和VIP可能参与IBS的病理生理过程。

Objective：The expression of Mast Cell（MC）and Vasoactive Intestinal Peptide（VIP）and in colonic mucosa in irritable bowel syndrome（IBS）patients,and its internal relations,for neural-endocrine-immune network analysis of the pathogenesis of IBS provides a theoretical basis.Methods：We selected the IBS patients of diarrhea-predominant IBS（D-IBS）,constipation-predominant IBS（C-IBS）patients and healthy controls each 20 cases.MC with toluidine blue staining,the determination of VIP with Immunohistochemical methods.Results：IBS patients was the more remarkably number of MC in both ileocecal,sigmoid colon mucosa than the normal control group（P0.01）,IBS patients were the more remarkably number of positive nerve fibers VIP in both ileocecal,sigmoid colon mucosa than the normal control group（P0.01）.Conclusions：MC and VIP might be involved in the pathophysiology of IBS.