不同强度静磁场对成骨细胞细胞内钙离子浓度的影响

Effect of static magnetic field at different strengths on Ca^(2+) concentration in rat primarily cultured osteoblasts

目的对成骨细胞静磁场加载过程中细胞内的钙离子浓度进行动态定量检测,探讨成骨细胞静磁场加载过程中细胞内钙离子浓度的变化规律。方法采用静磁场加载装置,对体外培养的成骨细胞分别在8、50mT和160mT的磁场强度下给予24、48h和72h的静磁场加载;0mT作为对照组,不进行静磁场加载,只受地磁场的影响。应用邻甲酚酞络合铜(OCPC)微板法测量加载后细胞内钙离子浓度。结果原代培养的细胞经鉴定具有成骨细胞的生物学特征,为成骨细胞。与0mT组相比,8、50mT和160mT的静磁场加载后24、48、72h成骨细胞内的钙离子浓度均降低(P<0.05)。与0mT组比较,8mT组在24、48、72h细胞内钙离子浓度降低率分别为27.48%、24.41%和10.46%,50mT组降低率分别为19.03%、18.04%和15.54%,160mT组降低率分别为8.58%、13.10%和19.03%。结论一定强度的静磁场加载能够降低成骨细胞内的钙离子浓度。

Objective To investigate the Ca2＋ concentration in osteoblasts and to study its changing rule during static magnetic field loading. Methods Osteoblasts primarily cultured form 1-day rat skull were cultured under 0, 8, 50 and 160 mT of static magnetic field for 24, 48 and 72 h, respectively. The cells cultured under 0 mT used as control. Osteoblasts were identified with alkaline phosphatase （ALP） and Von Kossa stainings. Ca2＋ concentrations were measured with the OCPC micro-plating method and analyzed by variance. Results The culuted cells showed the biological features of osteoblasts and positive to ALP and Von Kossa stainings. Compared with the cells under 0 mT field, the Ca2＋concentration of 8 mT group was decreased by 27.48%, 24.41% and 10.46% respectively at 24, 48 and 72 h, that of 50 mT group was by 19.03%, 18.04% and 15.54%, and that of 160 mT group was by 8.58%, 13.10% and 19.03% respectively for different time points. Significant reduce was found in the Ca2＋concentration after static magnetic field exposure compared with that of normal control （P0.05）. Conclusion The static magnetic field at a certain strength can decrease the Ca2＋concentration in osteoblasts.