人小细胞肺癌多药耐药细胞H446/CDDP基因组DNA甲基化状态的变化

Genomic DNA methylation in multidrug-resistant cell line H446/CDDP of human small-cell lung cancer

目的探讨基因组DNA甲基化状态与人小细胞肺癌(small cell lung cancer,SCLC)H446/CDDP细胞获得性多药耐药的关系。方法分别提取人SCLC多药耐药细胞H446/CDDP及其亲代细胞H446的基因组DNA,应用甲基化敏感性随机引物PCR(MS-AP-PCR)方法检测其甲基化状态。结果 H446/CDDP细胞经酶解后的扩增谱带较H446细胞明显增多,且荧光强度较强,表明其基因组DNA甲基化水平较亲代细胞明显增高。结论 H446/CDDP细胞多药耐药性的获得可能与其基因组DNA甲基化水平增高有关。

Objective To study the correlation between genomic DNA methylation and acquired multidrug-resistance of human small cell lung cancer （SCLC） cell line H446/CDDP. Methods Genomic DNA was extracted from H446/CDDP cells and their parental cells H446. Genomic DNA methylation was detected by methylation-sensitive arbitrarily primed PCR （MS-AP-PCR）. Results The amplified bands of H446/CDDP cells were significantly more than those of H446 cells and their fluorescence intensity was higher than that of H446 cells after enzymolysis, indicating that the genomic DNA methylation level is higher in H446/CDDP cells than in H446 cells. Conclusion The acquired multidrug-resistance of H446/CDDP cells is correlated with the high genomic DNA methylation level.