FSHβ亚基基因与繁殖性能及胎盘性状的关联分析

Association of Polymorphism for Porcine FSHβ Subunit Gene with Reproductive Traits and Placental Traits in Large White

以117头大白猪为研究对象,以FSHβ亚基基因为候选基因,采用PCR产物直接电泳方法检测FSHβ亚基基因位点的多态性,检出3种基因型,分别为AA、AB和BB基因型。将基因座不同基因型与总产仔数(TNB)、产活仔数(NBA)、初生体质量(BW)和胎盘性状(PT)进行关联分析。结果表明:FSHβ亚基基因在该位点上,就初产母猪而言,AA和AB基因型比BB型个体的TNB分别多3.25和2.37头(P<0.05),AA和AB基因型比BB型个体的NBA分别多3.31和2.49头(P<0.05)。就经产母猪而言,AA和AB基因型比BB型个体的TNB分别多2.71和1.60头(P<0.05),AA和AB基因型比BB型个体的NBA分别多2.95和1.74头(P<0.05)。AA基因型比AB和BB型个体的胎盘效率(PE)分别高16.28%和23.09%(P<0.05)。总体而言,FSHβ亚基基因在该位点的A等位基因对TNB、NBA和PE均表现为正效应。

FSHβ subunit gene was regarded as a candidate gene for reproductive traits in this study,and the polymorphisms of FSHβ was detected by electrophoresis method in 117 Large White pigs.Meanwhile,the effect of polymorphisms of FSHβ subunit gene on total born number（TNB）,number born alive（NBA）,birth weight（BW） and placental traits（PT） were analyzed.The results showed,about FSHβ locus,the first parity sows with genotype AA and AB had 3.25 and 2.37 TNB more than those with genotype BB.The first parity sows with genotype AA and AB had 3.31 and 2.49 NBA more than those with genotype BB,respectively.The multiparous sows with genotype AA and AB had 2.71 and 1.60 TNB more than those with genotype BB.The multiparous sows with genotype AA and AB had 2.95 and 1.74 NBA more than those with genotype BB.In multiparous sow population,the placental efficiency（PE） with AA genotype were higher than those with AA and BB genotypes significantly（P0.05） with 16.28% and 23.09%.The results of combined genotype effective indicated that A allele at FSHβ locus in all population had the positive effective on TNB,NBA and PE.