包裹钆对比剂的PLGA微球研究初步

Studies on Microparticles Enwrapping Gadolinium-based Contrast Agents

目的:制备包裹钆对比剂的PLGA微球,并评价PLGA微球在磁共振成像中的肾靶向增强效果。方法:Gd-DTPA作为内水相,溶入了PLGA的二氯甲烷有机溶剂作为油相,PVA-0486的水溶液作为外水相,采用复乳法制备包裹钆对比剂的PLGA微球;通过激光粒度分析仪测量微球平均粒径,用等离子体原子发射光谱仪测量微球载药量,并用磁共振成像仪观察微球的肾靶向增强效果。结果:PLGA浓度越大,PVA的浓度越小,合成的PLGA微球的平均粒径越大;PLGA微球对小鼠肾脏有明显的靶向增强作用。结论:包裹钆对比剂的PLGA微球有望成为一种能临床应用于磁共振成像的肾靶向对比剂。

Objective：To prepare of PLGA microparticles enwrapping Gadolinium-based contrast agents,and evaluate of the renal-targeting enhancement effect of these PLGA microparticles in the MRI imaging.Methods：PLGA microparticles enwrapping Gadolinium-based contrast agents were prepared by double emulsion solvent evaporation technique： Gd-DTPA solution as the inner water phase,methylene dichloride orgamic solvent containing PLGA as the oil phase,and PVA-0486 solution as the external water phase.The average diameter of these PLGA microparticles was scaled by Laser particle sizer,the drug loading was scaled by ICP-AES and the image enhancement was testified by MRI instrument.Results：The average diameter of the synthesized PLGA microparticles will become bigger while the concentration of PLGA increases or the concentration of PVA decreases;PLGA microparticles obviously enhance the renal of the mouse in the MRI imaging.Conclusions：PLGA microparticles enwrapping Gadolinium-based contrast agents may be used as a kind of renal-targeting MRI contrast agents in clinic.