内皮祖细胞在骨组织工程中的应用

Application of endothelial progenitor cell in bone tissue engineering

内皮祖细胞(endothelial progenitor cells,EPCs)可以来源于骨髓、脐带血、外周血及胎儿肝脏,其丰富的来源、较强的增殖能力及其参与血管发生(vasculogenesis)和血管新生(angiogenesis)的特性能够满足组织工程对种子细胞的要求,已成为构建血管化组织工程骨的新生力量。但目前来看,EPCs在骨组织工程中的应用研究较少,该文就目前有关EPCs的来源、分离与培养、鉴定、诱导与分化和在骨组织工程中的应用现状作一综述。

Endothelial progenitor cells can be derived from bone marrow,umbilical cord blood,peripheral blood and fetal liver,it is a kind of precursor cell which can differentiate into mature endothelial cells under the action of growth factor,and it is also known as the hemangioblast.EPCs are believed to home to sites of neovascularization,where they contribute to vascular regeneration by forming a structural component of capillaries and by secreting angiogenic factors.Its wide origin,strong proliferative capacity,vasculogenesis and angiogenesis abilities enable it to meet the requirements of seed cells for tissue engineering,in particular,it has become a new force in research of vascularization of tissue-engineered bone;however,there are few researches about EPCs applied in the bone tissue engineering at present,the source,separation and cultivation,identification,induction and differentiation of EPC and its application in bone tissue engineering are reviewed in this article.