3种梅毒血清学试验方法选择的临床分析

Clinical analysis of three kinds of syphilis serological test methods

目的比较常用的3种梅毒血清学试验方法,选择最合适的梅毒筛查的方法。方法采用目前临床实验室检测梅毒最常用的梅毒血浆快速反应素试验(RPR)、梅毒特异性抗体酶联免疫吸附试验(TP-ELISA)、梅毒螺旋体抗体血球凝集试验(TPPA)3种方法对住院及门诊患者进行梅毒血清学筛查。结果 3种试验方法阳性率中TP-ELISA法与TPPA法检测结果差异无统计学意义,而RPR法检测结果与TP-ELISA法和TPPA法比较差异均有统计学意义(P<0.01)。结论实验室应合理选用3种不同方法进行梅毒血清学检测,将减少漏诊和误诊率,为临床对梅毒患者的正确诊断提供参考依据,并且在判定梅毒的临床发展、分期、痊愈及药物疗效方面都具有十分重要的意义。

Objective To explore common three syphilis serologic test methods,so to select the most appropriate screening method of syphilis. Methods The syphilis serological screening of the clinic patients was conducted lay means of most commonly rapid plasma reagin （RPR）,syphilis antibody specificity enzyme-linked immunosorbent test （TP-ELISA） and Treponema pallidum particle agglutination （TPPA） three methods for hospital,which are very popular in the clinical laboratory for syphilis test. Results By the three kinds of test methods,there was no significant differences in TP-ELISA and TPPA method detection result compared with the one of RPR is of great significant difference （P 0.01）. Conclusion The laboratory shall reasonably select three different methods for syphilis,which will help to reduce the misdiagnosis rate of syphilis,and provide a reference basis for the identification of the development of syphilis in clinic,moreover,it is of great significance in the judgement of syphilis installment,healing and drug efficacy.