人类白细胞抗原B27表达及免疫学指标的分析在强直性脊柱炎的临床意义

Analysis of the clinical value of the expression and immune function of HLA-B27 antigen in the patients with ankylosing spongylitis

目的探讨人类白细胞抗原B27(HLA-B27)与强直性脊柱炎(AS)患者机体免疫功能的关系。方法以ECD标记的抗CD3单克隆抗体和PE-Cy5标记的CD4、CD8单克隆抗体作双色流式细胞术分析AS患者的CD3+ CD4+、CD3+ CD8+ T细胞百分率,免疫球蛋白、C-反应蛋白的检测采用速率散射比浊法法,抗链球菌溶血素O(ASO)采用乳胶凝集法测定。结果 HLA-B27抗原阳性患者各项免疫指标显著低于健康者,差异有统计学意义(P<0.01);HLA-B27阴性的患者各项免疫指标也显著低于健康者,差异有统计学意义(P<0.05)。结论 HLA-B27抗原在AS发病过程中起了重要作用,并对机体的免疫功能产生了一定影响。

Objective To study the relationship between HLA-B27 antigen and the immune function of the patients with ankylosing spongylitis.Methods The percentages of CD4＋ and CD8＋ and T cells were analyzed by flow cytometry （FCM） in vitro. Immunoglobulin and C-reactive protein （CRP） were measured by nephelometry immunoassay. Latex agglutination test was used to determine the titers of anti-streptococcal antibody （ASO）.Results The immune function showed great difference in AS patients with HLA-B27 expression,compared with that of healthy subjects. On the contrast,the immune function in AS patients without HLA-B27 expression showed no difference,compared with that of healthy subjects.Conclusion HLA-B27 antigen plays an important role in the pathogenesis of AS and affects human immune function.