刺梨四倍体诱导及其ISSR分析

Tetraploid Induction and ISSR Analysis in Rosa roxburghii Tratt

以刺梨为试材,利用组织培养结合秋水仙素溶液浸泡法诱导刺梨多倍体,并对四倍体与二倍体进行ISSR分析。结果表明:刺梨诱导芽分化增殖的最适培养基为MS+6-BA0.5mg/L+IAA0.1mg/L,诱导率达到100%,增殖系数4.4以上。用0.2%的秋水仙素溶液处理48h诱导率最高,纯合体诱导率为24.5%。四倍体与二倍体ISSR结果显示扩增条带差异明显,表明诱导加倍过程中四倍体发生了基因组变异。

Using Rosa roxburghii Tratt.as the experimental material,carried on the culture in vitro and using the colchicin solution inducted polyploidy,and analysized genomic defferences of tetraploid and diploid by ISSR.The results indicated that the most suitable culture medium which the Roxburghii induction differentiation multiplies was MS＋6-BA 0.5 mg/L＋IAA 0.1mg/L,the inductivity had achieved 100%,the k-factor was above 4.4.And the highest inductivity with 0.2%colchicin solution treatment 48h,the pure synthesis inductivity was 24.5%.The tetraploid and diploid ISSR result showed that expands banding was obvious difference,indicated that tetraploid has had the genome team variation in the induction of double process.