西藏野生灵芝粗多糖提取工艺研究

Study on Crude Polysaccharide Extraction Technology of Tibet Wild Ganoderma lucidum

采用正交试验,对影响灵芝多糖提取的4个主要因素(料水比、提取温度、提取时间、醇析浓度)进行了最佳工艺条件研究。结果表明,提取灵芝子实体粗多糖采用水提醇沉法,最优工艺条件为:料水比1∶50,提取温度90℃,提取2次,每次提取2h,90%乙醇醇析,3000r/min离心30 min取沉淀,sevage法除游离蛋白,离心取上层液即为灵芝粗多糖溶液。苯酚-硫酸法测定灵芝子实体粗多糖含量约为0.734%,这一含量在全国灵芝子实体多糖含量中处于中等水平,还有待于进一步的研究和开发利用。

The ganoderan is the main active ingredient of the Ganoderma lucidum, which content has become an important indicator to measure its quality. Using water extraction and alcohol precipitation method extracts crude polysaccharide of the mythic fungus germentity, the optimal conditions analyzed orthogonal experiment method is followed below： ratio ofmaterial to water is 1∶50, the extraction temperature is 90℃, and extracting for twice per 2 h, 90% alcohol analysis, centrifuging for 30 m at 3 000 rpm and take precipitation, using the way of sevage to remove floating preteins, centrifugation and taking the upper liquid which is the G. lucidum crude polysaccharide liquid. It is using the way of Phenol-sulfuric acid method to measure the contents of the crude polysaccharide of the mythic fungus germentity which is 0.734%, andit is at upper middle level at polysaccharide of the mythic fungus germentity nationwide, it is also demanding further research and development and use.