甘油果糖对宫内窘迫胎鼠脑保护作用的研究

Study on protective effect of glycerol fructose for brain tissues of fetal rats with intrauterine fetal distress

目的:研究甘油果糖注射液对宫内缺血缺氧胎鼠脑组织自由基和细胞凋亡的影响,探讨甘油果糖对宫内窘迫胎鼠脑组织的保护作用。方法:Wistar雌性大白鼠,于妊娠第19天通过无损伤动脉钳钳夹双侧子宫、卵巢血管20 min建立缺血缺氧宫内窘迫模型。孕鼠随机分为假手术组(A组),缺血缺氧组(B组),甘油果糖治疗组(3.5 ml/100g,腹腔注射):甘油果糖缺血缺氧(HI)前30 min治疗组(C组)和HI后立即治疗组(D组)。此后实验用母鼠均关腹放回原饲养环境中24 h后,立即剖宫取胎,确定胎鼠是否存活,在每组存活胎鼠中随机抽取10只断头取脑,以比色法测定脑组织丙二醛(MDA)含量、TUNEL法观察脑海马区神经元凋亡。结果:①与B组比较,C组、D组胎鼠死亡率明显降低(P<0.05)。②与B组比较,C组、D组脑组织MDA含量降低(P<0.01,P<0.05);与D组比较,C组脑组织MDA含量降低(P<0.01)。③与A组比较,B组脑海马神经元凋亡数目明显增加(P<0.01);C组、D组与B组比较,海马区凋亡细胞数目减少(P<0.01,P<0.05);C组凋亡细胞数目多于D组(P<0.01)。结论:①实验证明甘油果糖能够通过胎盘屏障及血脑屏障,可能通过促进能量代谢途径对缺血缺氧性脑损伤(HIBD)胎鼠起脑保护作用。②实验表明缺血缺氧性脑损伤前用药比其后用药效果更佳。

Objective： To study the effects of glycerol fructose injections on free radical and apoptosis of brain tissues of fetal rats with intrauterine hypoxic - ischemic injury, explore the protective effect of glycerol fructose for brain tissues of fetal rats with intrauterine fetal distress： Methods： Wistar female rats model of intrauterine hypoxicischemic injury were established by blocking bilateral uterine vessels and ovarian vessels for 20 minutes with non - traumatic artery forceps on the nineteenth day during pregnancy ; the pregnant rats were divided into sham operation group （group A）, hypoxic -ischemic group （group B） and glycerol fructose group [3.5 ml/10Og, intraperitoneal injection, including the first 30 minutes glycerol fructose group （group C） and immediate glycerol fructose group （group D）] ; all the pregnant rats were reared for 24 hours after abdomen - closing, then the fetal rats were obtained by cesarean section to determine that they were alive or not, 10 alive fetal rats were selected randomly, calorimetric method was used to detect the content of malondialdehyde （MDA）, the apop- tosis of neurons in hippocampus of fetal rats was observed by TUNEL method. Results： Compared with group B, the mortalities of group C and group D decreased significantly （ P 〈 0. 05 ） . Compared with group B, the contents of MDA in group C and group D decreased significantly （ P 〈 0. 01, P 〈 0. 05 ） . Compared with group D, the content of MDA in group C decreased significantly （ P 〈 0. 01 ） . Compared with group A, the number of cerebral hippocampus apoptosis in group B increased significantly; compared with group B, the numbers of cer- ebral hippocampus apoptosis in group C and group D decreased significantly （ P 〈 0. 01, P 〈 0. 05 ） ; the number of cerebral hippecampus apoptosis in group C was significantly higher than that in group D （P 〈 0. 01 ） . Conduslon： Glycerol fructose can pass placental barrier and hematoencephalic barrier, which may play a protective effect for fetal rats with hypoxic - ischemic brain damage （HIBD） by promoting energy metabolism; preventive medication before HIBD is better than medicine intervention after HIBD.