睡眠剥夺对模拟高原环境下高原肺水肿形成的影响

Effects of sleep deprivation on development of high altitude pulmonary edema in a simulated high altitude environment in rats

目的探讨睡眠剥夺对模拟高原环境下高原肺水肿(high altitude pulmonary edema,HAPE)形成的影响,为防治HAPE提供理论依据。方法雄性SD大鼠,随机分成4组,即平原对照组、高原对照组、高原剥夺组、平原剥夺组,睡眠剥夺采用小平台水环境法建立,高原环境用5000 m低压氧舱模拟。通过测定大鼠肺湿/干比值、肺含水比值、动脉血气来评定肺水肿的程度,并通过大鼠肺组织石蜡切片观察各组病变。结果高原剥夺组的肺湿/干比值及肺含水量比值显著高于其余3组(P<0.01)。高原剥夺组可见肺间质增厚、肿大,大量多核细胞,肺泡腔变小,肺泡内无液体渗出,其他组病理切片未见上述变化。结论高原环境下睡眠剥夺可促发急性HAPE的形成可能与交感神经活性增强有关。

Objective To investigate the impacts of sleep deprivation on the development of high altitude pulmonary edema（HAPE） in a simulated high altitude environment in rats.Methods 44 male SD rats were randomly divided into plain control group,plateau control group,plateau sleep deprivation group,plain sleep deprivation group.The small platform method was used to induce sleep deprivation,and a hypobaric oxygen chamber was used to simulate the high altitude environment of 5000 m above sea level.The degree of pulmonary edema was assessed by measuring lung wet/dry weight ratio,lung water ratio and arterial blood gas.The paraffin sections of lung tissue were made to observe the pathological changes in each group after different treatments.Results The lung wet/dry weight ratio and water ratio in plateau sleep deprivation group was significantly higher than that in other three groups（P0.01）.Moreover,the lung sections showed that thickened and swollen pulmonary interstitium,a large number of multinucleated cells and smaller alveolar space without leaking liquid were observed in plateau sleep deprivation group.These changes were not found in other groups.Conclusion Sleep deprivation can trigger the acute episode of HAPE at high altitude,which may be related to the enhancement of sympathetic activity.