转基因“超级鲤”的构建

Production of Transgenic “Super Common Carp”

用显微注射的方法共生产出以大麻哈鱼生长激素基因为结构基因，鲤鱼ＭＴ基因为启动子的融合基因转基因鲤鱼１．５万尾，同时筛选生长快表型性状好的个体进行ＤｏｔＢｌｏｔ和ＳｏｕｔｈｅｒｎＢｌｏｔ杂交，得到１８９尾整合外源生长激素基因鲤鱼，并获得１尾比对照组最大个体体重大１．８倍的“超级鲤”。通过年龄鉴定确认其为３龄鱼。“超级鲤”

Fifteen thousand transgenic common carp which were transferred all fish gene(promoter of common carp metalloghionein and growth hormone gene of Salmon) were got by micro injection method and 189 transgenic common carp having integrated foreign growth gene into genome were gained by Dot and Southern Blot hybridization. A “super common carp” whose body weight is 1.8 times greater than the biggest individal of the control was obtained. It is realized to be three year old through age identification.