温度对神仙鱼主要消化酶活力的影响

The Effect of Water Temperature on Main Digestive Enzymes' Activeness of Pterophyllum Scalare

在不同的反应温度和不同的饲养水温条件下,研究神仙鱼3种主要消化酶的活性。结果表明,在pH7.5、反应时间10min时,蛋白酶活性的最适反应温度为45℃;在pH6.9、反应时间5min时,淀粉酶的最适反应温度为40℃;在pH7.5、反应时间60min时,脂肪酶的最适反应温度为30℃。在16～25℃养殖水温条件下,3种酶的活性随水温的降低而降低。蛋白酶和脂肪酶的最适水温均为25℃,但均与22℃时的差异不显著(P>0.05);淀粉酶活性的最适水温为22℃,但与25℃时的差异不显著(P>0.05)。

In this paper,the effect of the temperature on the digestion of Pterophyllum scalare is studied.The results are as follows：1.In 10 min reaction time with pH7.5,the activeness of protease＇s most appropriate reaction temperature measured in Pterophyllum scalare is 45℃.In 5 min reaction time with pH6.9,the most appropriate reaction temperature of amylase measured in Pterophyllum scalare is 40℃.In 60 minutes reaction time with pH7.5,when the most appropriate reaction temperature of lipase measured in Pterophyllum scalare is 30℃.2.When the temperature condition of the culture water is between 16℃ to 25℃,the activeness of lipase and protease in Pterophyllum scalare decreases with temperature.Their most appropriate reaction temperature is 25℃,which has little difference with that of 22℃（P 0.05）.The activeness of amylase＇s most appropriate reaction temperature is 22℃,which has little difference with that of 25℃（P 0.05）.Thus,when the water temperature is 22℃,it has little influence on the digestion of Pterophyllum scalare.