HPLC法同时测定复方甘草酸苷片中甘氨酸和蛋氨酸的含量

Determination of glycine and methionine in Compound Glycyrrhizin Tablets by HPLC

目的建立HPLC法同时测定复方甘草酸苷片中甘氨酸和蛋氨酸的含量。方法采用AgilentHC-C18(2)色谱柱(4.6 mm×250 mm,5μm),乙腈-0.05 mol/L磷酸二氢钾溶液(50%磷酸调节pH值至2.5)(5∶95)为流动相;流速:0.8 mL/m in;检测波长:200 nm;柱温:30℃。结果甘氨酸与蛋氨酸均在0.10～1.0 mg/mL的浓度范围内有良好的线性关系(r=1.000 0和0.999 9);平均回收率分别为100.29%和99.58%;RSD分别为0.97%和0.75%(n=9)。结论此法简便、快速、准确,适用于同时测定复方甘草酸苷片中甘氨酸和蛋氨酸的含量。

Purpose To establish a HPLC method for determination of glycine and methionine in Compound Glycyrrhizin Tablets.Methods The HPLC method was performed on a Agilent HC-C18（2）column（4.6 mm×250 mm,5 μm）.The mobile phase was composed of acetonitrile-0.05 mol/L KH2PO4（adjusting pH to 2.5 with 50% phosphate acid）（5∶95）.The flow rate was 0.8 mL/min.The detection wavelength was 200 nm.The column temperature was 30 ℃.Results Both glycine and methionine showed a good linear（r=1.000 0 and 0.999 9）in the range of 0.10-1.0 mg/mL respectlively.The average recoveies were 100.29% with RSD of 0.97% and 99.58% with RSD of 0.75%（n=9）.Conclusion The method might be simple,rapid,and accurate.It is suitable for determination of glycine and methionine in Compound Glycyrrhizin Tablets.