大肠癌组织中端粒酶活性P53及nm23的表达和临床意义的研究

Expression and clinical significance of telomerase activity,P53 and nm23 in colorectal carcinoma

目的探讨端粒酶活性、P53及nm23在原发性大肠癌组织中的表达和临床意义。方法应用免疫组织化学技术检测100例大肠癌P53和nm23的表达,同时应用原位杂交技术检测其中30例大肠癌及其癌旁组织、正常黏膜中端粒酶活性的表达,分析其与大肠癌的临床病理之间的关系。结果大肠癌组织中端粒酶阳性表达明显高于癌旁组织、正常黏膜组织(P<0.05),端粒酶表达强度与肿瘤的浸润深度、临床分期及淋巴结转移具有相关性(P<0.05)。P53阳性表达及强度与肿瘤的分化程度、临床分期显著相关(P<0.05)。P53表达与nm23低表达之间呈负相关(r=-0.115,P<0.05)。结论端粒酶的激活、p53基因突变及nm23基因缺失在大肠癌的发生发展中有一定的作用,联合检测端粒酶活性、P53、nm23对大肠癌的诊断、治疗及预后判断有意义,尤其P53与nm23同时检测更有价值。

Objective To evaluate expression and clinical significance of telomerase activity,P53 and nm23 in primary colorectal carcinoma.Methods The expression of P53 and nm23 were examined by immunohistochemical technique in 100 cases of colorectal carcinoma.Telomerase activity was examined by using in situ hybridization mRNA of telomerase.The relationship between the expression and clinical-pathological behaviors were analyzed.Results The positive expression of telomerase activity in cancer tissues was significantly higher than that in adjacent cancer tissues and in normal tissues（P〈0.05）.Telomerase activity level in colorectal carcinoma was significantly associated with the invasion,clinicopathologic stage and lymph node metastasis（P〈0.05）.The expression of P53 had a relevancy with the grade and clinical stage（P〈0.05）.A minor relationship was observed between P53 over-expression and nm23 low-expression（r=-0.115）.Conclusion The expression and level of telomerase activity,P53 gene and nm23 mutation play some roles in development of colorectal carcinoma.They might be a good sign and helpful in diagnosis,treatment and prognostic evaluation of colorectal carcinoma.The expression of P53 and nm23 examined simultaneously might be valuable.