非贵金属烤瓷冠对家兔牙龈组织的影响

Influence of base metal porcelain crown on hare gingiva tissue

目的研究家兔戴实验冠后牙龈组织中镍铬元素的含量及增殖细胞核抗原(PCNA)、bcl-2蛋白的表达。方法采用免疫组化和石墨炉原子吸收光谱法。结果戴冠3个月组镍铬元素含量与对照组无差异,6个月组比对照组及3个月组高,差异有统计学意义;3个月组PCNA与对照组表达比较差异有统计学意义,6个月组与对照组无差异。3、6个月组与对照组Bcl-2表达无差异。结论非贵金属烤瓷冠金属离子可游离于牙龈组织;PCNA表达与炎症反应有关;PCNA、bcl-2的表达,不表明牙龈组织有异常增殖。

Objective To study the content of Nickel（Ni）and Chromium（Cr）and the expression of PCNA、bcl-2 protein in the Hares gingiva tissue.Methods Immunohistochemistry S-P and atomic absorption spectrometer methed.Resusts The content of Nickel（Ni）and Chromium（Cr）had no significant difference between the 3 month group and the contrast group.It had significant difference between the 6 month group and 3 month group and the contrast group.The expression of PCNA had significant difference between the 3 month group and the contrast group,It had no significant difference between the 6 month group and the contrast group.The expression of bcl-2 had no significant difference between the 6 month group and the contrast group.Conclusion The metalion was released in gingiva tissue.There were a correlation between the expression of PCNA and inflammation of the gingiva tissue.The expression of PCNA and bcl-2 protein can not indicate the gingiva tissue to be dysplasia.