α-干扰素联合脂多糖在体外抑制急性髓系白血病细胞株U937和HL60增殖的效应

Inhibitory effects of IFN-α and lipopolysaccharide on proliferation of acute myeloma leukemia cell lines U937 and UL60 in vitro

目的探讨α-干扰素联合脂多糖在体外对髓系白血病(AML)细胞系U937、HL60细胞增殖、凋亡及细胞周期的影响。方法用四甲基偶氮唑蓝法检测α-干扰素和(或)脂多糖对U937、HL60细胞增殖作用的影响;流式细胞仪检测α-干扰素和(或)脂多糖对U937、HL60细胞的凋亡率和细胞周期的影响。结果 (1)单用α-干扰素或脂多糖组较对照组能显著地抑制U937、HL60细胞的增殖、增加细胞的凋亡率和增加G1期细胞(P<0.05)。(2)α-干扰素联合脂多糖组较单用α-干扰素或脂多糖组及对照组均能显著地抑制U937、HL60细胞的增殖、增加细胞的凋亡率和增加G1期细胞(P<0.05)。结论α-干扰素联合脂多糖对白血病细胞增殖抑制及凋亡促进有协同增强作用。

Objective To investigate the effects of IFN-α in combination with lipopolysaccharide on the cellular proliferation,apoptosis and cell cycle of acute myelogenous leukemia（AML）cell lines U937 and HL60 in vitro.Methods The effects of IFN-α and lipopolysaccharide on AML cell lines U937 and HL60 proliferation were detected by MTT.Cells apoptotic rate and cell cycle were evaluated by using annexin-V FITC/PI apoptotic kit and flow cytometry,respectively.Results（1）We found that IFN-α or lipopolysaccharide alone could inhibit leukemic proliferation,increase cellular apoptotic rate and percentage of cells at G1 phase significantly,comparing with the control group（P〈0.05）.（2）IFN-α in combination with lipopolysaccharide could inhibit leukemic proliferation,increase the cellular apoptotic rate and percentage of cells at G1 phase significantly,comparing with IFN-αor lipopolysaccharide alone and the control group,respectively（P〈0.05）.Conclusion IFN-α in combination with lipopolysaccharide has synergistic effect on the proliferation inhibition and cellular apoptotic induction of AML cells.