亚甲蓝光化学照射法可降低血浆IgM抗-A抗-B血型抗体效价

Effect of photochemical irradiation by methylene blue on the titers of anti-A and anti-B IgM antibody in plasma

目的了解血浆经亚甲蓝/光化学照射后血型IgM抗体效价与积分的变化。方法使用一次性血浆病毒灭活输血过滤器,将含亚甲蓝血浆的光照袋及滤器放入医用病毒灭活箱,灭活血浆病毒。比较光化学照射前后抗-A、抗-B抗体效价的变化。结果 A型血浆抗-B照射前后比较效价下降明显,差异均有统计学意义(P<0.004、积分P<0.001);B型血浆抗-A效价下降明显,差异有统计学意义(P<0.010)。结论血浆经亚甲蓝/光化学照射后,血浆血型抗体均受影响,活性降低。

Objective To investigate the change in the titer and scoring of the blood type IgM antibody in plasma after the photochemical irradiation by methylene blue.Methods One-off blood transfusion filter with plasma virus inactivation was employed.The filter and the illuminating bag containing plasma and methylene blue were placed in virus inactivation box for the killing of the virus in plasma.The changes in the anti-A and anti-B antibody titers before and after the photochemical irradiation were compared.Results The difference in anti-B antibody titer in type A plasma before and after the irradiation was obvious（P〈0.004）and the score difference was evident（P〈0.001）.But the difference in anti-A titer in type B plasma before and after the irradiation was remarkable（P〈0.010）and the score difference was obviously（P〈0.001）.Conclusion After the plasma was irradiated by methylene blue/ photochemical ray,the blood antibodies in plasma were significantly weakened.