温肾化石汤对草酸钙结石模型大鼠脂质过氧化的影响

Investigation of Wenshenhuashi decoction on lipid peroxide in rat with calcium oxalate calculus

目的探讨温肾化石汤对草酸钙结石模型大鼠脂质过氧化的影响。方法 SD雄性大鼠40只,随机分为4组:空白对照组、模型组、温肾化石汤高剂量组和温肾化石汤低剂量组,每组10只。除空白对照组外,其它各组大鼠喂标准鼠料,饮用含0.75%乙二醇和0.75%氯化铵的自来水。温肾化石汤高剂量组大鼠予温肾化石汤提取液10mL/kg(30 g/kg),每日灌胃1次,温肾化石汤低剂量组予温肾化石汤提取液稀释1倍后10 mL/kg(15 g/kg),每日灌胃1次。空白对照组、模型组按体质量灌胃等容积自来水每日1次。各组均饲养6周。处死大鼠,检测各组大鼠肾组织超氧化物歧化酶(SOD)、丙二醛(MDA)的含量,并观察肾组织结构的变化及草酸钙晶体的分布。结果温肾化石汤可明显升高肾组织SOD含量,降低MDA含量。草酸钙晶体形成程度、肾组织细胞的损伤程度明显减轻。结论温肾化石汤可以增强抗脂质过氧化的作用,保护肾脏组织结构,对草酸钙晶体形成有明显的抑制作用。

Objective To investigate the effect of Wenshenhuashi decoction on lipid peroxide in rat with calcium oxalate calculus.Methods 40 SD male rats were randomly divided into four groups,blank control group,model group,Wenshenhuashi decoction high dose group and Wenshenhuashi decoction low dose group.Except control group,rats in other groups were feed by standard feed and tap water with 0.75% ethylene and 0.75% ammonium chloride.Rats in Wenshenhuashi decoction high dose group were treated by extraction of Wenshenhuashi decoction,10 mL/kg（30 g/kg）,once a day.Rats in Wenshenhuashi decoction low dose group were treated by 1-fold diluted extraction of Wenshenhuashi decoction,10 mL/kg（15 g/kg）.The course was six weeks in each group.After six weeks,rats were executed and the contents of SOD and MDA in nephridial tissue of rat were measured.The changes of kidney structure and distribution of calcium oxalate crystal were observed.Results Wenshenhuashi decoction can increase SOD and decrease MDA.It can lessen formation of calcium oxalate crystal and injury of renal cell.Conclusion Wenshenhuashi decoction can enhance a role of anti-lipid peroxidation,protect kidney structure and inhibit the formation of calcium oxalate crystal.