桃红四物汤有效成分的交互作用及其对骨痂微血管形态学的影响

The interactions of Taohongsiwu Decoction’s effective constituents and the influences on the histomorphology and the micrangium morphosis of callus

目的:探讨桃红四物汤主次要有效成分的交互作用及其对骨痂组织及骨痂微血管形态的影响。方法:从桃红四物汤中提取了4类有效成分:总生物碱(A)、总苷(B)、总多糖(C)和总挥发油(D),按照前期实验所得结果(主要成分为A,次要部位为B、C、D),对清洁级小白鼠徒手定点折骨造模后进行实验。按照L8(27)正交设计方案分组,观察分析骨折断端骨痂组织形态、微血管新生形态,探讨主次要成分之间是否存在交互作用。结果:提取成分处方组与原方组药物能促进骨折后骨生成细胞、成骨细胞、骨痂微血管的生长,并能使骨新生和微血管新生提早进入塑形期,与模型组比较有统计学意义(P<0.05)。其中第1组对骨细胞与微血管新生、重建的促进作用最强。另从L8(27)正交实验方差分析法分析,总苷类取水平1和水平2差异有统计学意义(P<0.05),而总多糖和总挥发油类取水平1和水平2均无统计学意义(P>0.05)。结论:有效成分总生物碱与总苷有交互作用,与总多糖和总挥发油无交互作用;总苷、总多糖与总挥发油之间也无交互作用。

Objective：To explore the interactions of Taohongsiwu Decoction’s （THSWD’s） effective constituents and the influences on the histomorphology and the micrangium morphosis of callus.Methods：Based on the former experiment,we extracted 4 effective constituents from THSWD：A（total alkaloids TA） who is the principal,B（total glycosides TG）,C（total polysaccharide TPS） and D（volatile oil TVO） who is the secondary, executed experiment on the mus musculus albus after bare-handed fixed-point bone snaped, layed 8 groups according to the orthogonal design proposal, analyzed the histomorphology of callus and blood vessel regeneration,which in order to study on whether there is interactions on Taohongsiwu Decoction’s （THSWD’s） principal and secondary or not.Results：The groups of extraction and raw could all promot the development of osteoblast and blood capillary,and advanced shaping them,which was significantly more than that in the model group （P〉0.05）. Nevertheless the observation group was the best.The orthogonal experiment indicated that there was obvious difference between B’s level 1 and level 2（P〉0.05）.But there was no difference between C’s or D’s level 1 and level 2（P〉0.05）.Conclusion：there was interactions between TA and TC,but there was no interactions among TA,TPS and TVO,and so did among TC,TPS and TVO.