苏南地区丙型肝炎病毒基因型分布及其与NK细胞比例异常的关系

Distribution of Hepatitis C Virus Genotypes and its Response to Abnormal Ratio of NK Cells in South Jiangsu

目的①了解苏南地区丙型肝炎病毒的基因型分布。②测定丙型肝炎患者外周血中NK细胞的百分数。方法用基因芯片方法对103例丙型肝炎患者的血清进行病毒基因分型,并测定这103例丙肝患者外周血淋巴细胞中NK细胞的百分数。结果 103例丙型肝炎中1b型95例(92.2%),2a型4例(3.9%),3b型1例(1.0%),1b+2a混合型3例(2.9%),未发现1a、3a。103例丙型肝炎患者外周血中NK细胞的百分数低于正常对照组(P<0.01),不同丙肝基因型患者NK细胞的百分比无显著性差异(P>0.05)。结论①苏南地区丙肝患者基因分型以1b型为优势株。②NK细胞的减少是导致丙型肝炎的慢性化过程与迁延不愈的一个重要因素。

Objective To investigate the distribution of HCV genotypes in South Jiangsu and the percentage of NK cells among peripheral blood lymphocytes in patients with hepatitis C. Methods HCV genotyping was conducted in 103 cases with hepatitis C with the gene chip. The percentage of NK cells among peripheral blood lymphocytes was also detected. Results There were 95 cases with type 1b（92.2%） ,4 cases with type 2a（3.9%） ,1 case with type 3b（1.0%） ,and 3 cases with type 1b ＋ 2a（2.9%） in 103 cases with hepatitis C. The percentage of NK cells among peripheral blood lymphocytes in patients with hepatitis C was lower than that in the normal control group（P 0.01） and there was no significant difference in the percentages of NK cells in patients with different genotypes（P 0.05） . Conclusion Most patients with hepatitis C are genotyped as type 1b. So type 1b virus is the dominant strain in South Jiangsu. Decreased activity and reduction of NK cells is an important factor which leads to the chronic and protracted process of hepatitis C.