表皮葡萄球菌ica操纵子与聚氯乙烯材料表面细菌生物膜形成的关系

Relationship between ica Operon of Iatrogenic Staphylococcus epidermidis and Formation of Bacterial Biofilm on Surface of Polyvinyl Chloride

目的分析医源性表皮葡萄球菌生物膜基因型,探讨医源性表皮葡萄球菌ica操纵子与聚氯乙烯(PVC)材料表面细菌生物膜形成的关系。方法应用PCR法、DNA凝胶成像及基因测序技术检测医源性表皮葡萄球菌的生物膜形成相关基因,采用半定量测定方法测定PVC材料表面表皮葡萄球菌的细菌生物膜形成能力。结果医源性表皮葡萄球菌16S rRNA阳性率为100.0%;icaADB、atlE、fbe阳性基因型菌株占57.1%;icaADB阴性,atlE、fbe阳性基因型菌株占37.5%;ica操纵子阳性表皮葡萄球菌在PVC材料表面的细菌生物膜形成能力较ica操纵子阴性明显增强,差异有统计学意义(P<0.01)。结论医源性表皮葡萄球菌可以分为ica操纵子阴性和ica操纵子阳性两类细菌,ica操纵子可以增加PVC材料表面细菌生物膜的形成能力,这可能是区分共生性和侵袭性表皮葡萄球菌的遗传基础。

OBJECTIVE To analyze the genotype of iatrogenic Staphylococcus epidermidis（SEP） and explore the relationship between its SEP ica operon and the formation of bacterial biofilm on the surface of polyvinyl chloride（PVC）.METHODS The genotype of iatrogenic SEP was detected by the application of PCR method,DNA gel imaging and gene sequencing,and the capability of biofilm formation of SEP on the surface of PVC material was determined by semi-quantitative method.RESULTS The positive rate of 16S rRNA of iatrogenic SEP strains was 100.0%.The genotypes of icaADB＋,atlE＋ and fbe＋ strains accounted for 57.1%.The genotype of icaADB negative but atlE and fbe positive strains accounted for 37.5%.The capability of biofilm formation of ica operon positive strains of SEP on the surface of PVC material was more strong than that of ica operon-negative strains（P0.01）.CONCLUSION Iatrogenic SEP can be divided into two types of ica operon negative and ica operon-positive bacteria.Iatrogenic SEP with positive ica operon can increase the capability of biofilm formation on the surface of PVC material the.It may be the genetic basis of iatrogenic SEP to distinguish between symbiosis and aggressive strains.