荧光定量聚合酶链反应对女性生殖道感染解脲脲支原体及沙眼衣原体检测分析

Fluorescence Quantitative PCR for Detection of Ureaplasma urealyticum and Chlamydia trachomatis in Female Reproductive Tract Infections

目的了解女性生殖道感染者解脲脲支原体（Uu）和沙眼衣原体（Ct）的感染情况,为临床诊断与治疗提供试验依据。方法对374例女性生殖道感染者按常规取宫颈或阴道分泌物,利用荧光定量聚合酶链反应（FQ-PCR）方法分别进行Uu和Ct检测。结果 374例生殖道感染女性中Uu感染率61.7%,Ct感染率6.6%,Uu和Ct混合感染率6.0%;将患者按年龄分为18~20、21~30、31~40、41~50、〉50岁5个组,其Uu和Ct检测阳性率分别为72.7%和50.0%、62.7%和5.6%、62.0%和5.4%、28.6%和0、0和0。结论在女性生殖道感染常见病原体中Uu感染率明显高于Ct,而且Uu多以单独感染形式为主,Ct多以混合感染为主,两种病原体的感染率都随着年龄的递增有递减的趋势;对生殖道感染女性常规检测Uu和Ct,对指导临床治疗具有重要意义。

OBJECTIVE To observe the infection statas of Ureaplasma urealyticum（Uu） and Chlamydia trachomatis（Ct） in females with the reproductive tract infections,to provide experimental evidences for clinical diagnosis and therapy.METHODS Fluorescence quantitative polymerase chain reaction（FQ-PCR） was performed on 374 cervical or vaginal secretions obtained from women with genital tract infections for Ct and Uu detection.RESULTS The results of 374 cases of reproductive tract infections in women showed that Uu infection rate was 61.7%,Ct infection rate was 6.6%,and Uu and Ct mixed infection rate was 6.0%.The patients were divided into five groups：18 to 20 years old,21 to 30 years old,31 to 40 years old,41 to 50 years and more than 50 years old,the positive rates of Uu and Ct were 72.7% and 50.0%,62.7% and 5.6%,62.0% and 5.4%,28.6% and 0,0 and 0,respectively.CONCLUSION In the female reproductive tract infections,Uu infection is markedly higher than Ct,and Uu infection is mainly in the form of single infection,Ct mostly dominates mixed infection.The infection rate of two kinds of pathogens are increasing with the age.These results show that the routine detections of Uu and Ct for reproductive tract infections have a great significance for the diagnosis and treatment of the female genital tract infection.