噻唑蓝法测定抗肿瘤复方制剂仙慈丹的体外抗肿瘤活性

Determination of Anti-Tumor Activity of Compound Preparation Xiancidan in Viro by MTT Method

目的分离筛选出抗肿瘤复方制剂仙慈丹(XCD)的抗肿瘤活性部位。方法采用大孔树脂柱色谱法对抗肿瘤复方制剂XCD水提取液进行分段富集,运用噻唑蓝(MTT)比色法对分段富集得到的部位进行体外抗肿瘤活性测定。结果抗肿瘤复方制剂XCD水提取液过AB-8型树脂纯化得到的P2(体积分数20%乙醇洗脱液)、P3(体积分数40%乙醇洗脱液)、P4(体积分数60%乙醇洗脱液)、P5(体积分数80%乙醇洗脱液)对人肝癌HepG2细胞表现出较强的抑制作用。结论 XCD水提液过大孔树脂后乙醇洗脱部位是XCD制剂的抗肿瘤活性部位。

OBJECTIVE To separate and select anti-tumor activity site of the anti-tumor compound preparation Xiancidan（XCD）.METHODS The means of macroporous resin column chromatography（AB-8） was used to separate the water extract from Chinese drug XCD.The anti-tumor activities of the different extracts in vitro were analyzed with MTT method.RESULTS P2（20% ethanol elution）,P3（40% ethanol elution）,P4（60% ethanol elution） and P5（80% ethanol elution） strongly inhibited cell proliferation of HepG2.CONCLUSION Ethanol elution position is the anti-tumor extract from XCD.