两株假单胞菌降解酚类化合物的特性

Characteristics of degradation of phenolic compounds by two Pseudomonas strains

从焦化废水中分离得到2株可降解对氯酚的假单胞菌(Pseudomonas)CO-1和CO-44.其最适降解温度为35～40℃,最适pH值为6.0～8.0.菌株降解对氯酚的速度与对氯酚初始浓度呈负相关.2株细菌均能较快地降解苯酚和甲酚,其中CO-1还能够降解1-萘酚和萘.在添加对氯酚的焦化废水中,CO-1和CO-44能够在42h内将苯酚、甲酚和对氯酚完全降解.从2株细菌中均检测到了苯酚羟化酶基因,分别从菌株CO-1和CO-44中检测到邻苯二酚1,2-双加氧酶基因和邻苯二酚2,3-双加氧酶基因.

Two bacterial strains, Pseudomonas sp. CO-1 and CO-44, capable of degrading 4-chlorophenol, were isolated from coking wastewater collected from Shaoguan, Guangdong, South of China. The optimum conditions for 4-chlorophenol degradation by these two strains were pH 6.0~8.0 and temperatures 35~40 ℃. The degradation rate of 4-chlorophenol was negatively related with the initial concentration of 4-chlorophenol in mineral salt medium. Both strains could degrade phenol and cresols, strain CO-1 also could degrade 1-naphthyl phenol and naphthalene. Approximately 300mg/L phenol and 100mg/L cresols in coking wastewater as well as 100mg/L 4-chlorophenol supplemented were completely degraded within 42 hours at 2% inoculum rate. Phenol hydroxylase gene were detected from both two strains, in addition to catechol 1,2-dioxygenase gene and catechol 2,3-dioxygenase gene were detected from strain CO-1 and CO-44 respectively.