油田土壤微生物群落碳代谢与理化因子关系研究

Studies on relationship between carbon metabolism of soil microbial community and soil physicochemical factors

基于对我国主要油田区的土壤理化性质等场地信息调查,应用Biolog和统计学方法分析理化因子与土壤微生物群落碳代谢特性的关系,揭示了不同区域油田土壤理化因子对微生物群落代谢的综合影响作用.结果表明:油田区土壤理化特性呈现地理性分布特性;总氮、总磷、速效磷、有效态铜、锌等环境因子与微生物群落的多样性正相关;可溶盐含量、pH值、总氮、总有机碳等对样点间微生物群落碳代谢的差异性贡献最大.对各油田碳代谢影响因子比较表明,不同区域的油田区土壤中影响微生物群落代谢特性的主要因子各不相同,同一理化因子在不同油田区对微生物群落碳代谢的影响程度不同.这种代谢特性与理化因子关系的区域性差异可能是土壤物化性质地理性分布以及微生物群落结构区域性差异共同作用的结果.

Based on the investigation of geochemical characteristics of main oil fields in China, Biolog and statistical approaches were employed to analyse the relationship between soil physicochemical factors and carbon metabolism of soil microbial community. The results revealed the complex effects of geochemical properties of oil fields on microbial community in the different geographic regions. The soil physicochemical properties had geographic distribution. Total nitrogen and phosphorus, available nitrogen, available copper and zinc showed positive correlation with the diversity of microbial community. Soluble salt, pH, total nitrogen, and total organic carbon were the main factors that influenced the carbon source utilization. The results indicated that the main factors which affect carbon metabolism were site-specific for the various regions. Additionally, a certain factor could exhibit different influences on the microbial community in the various oil fields. The site-specific relationships between regional soil physicochemical properties and microbial metabolism were attributed to the common effects of the geographic distribution of physicochemical properties and the regional difference of original microbial community structure.