黑曲霉产壳聚糖发酵工艺的优化

Optimization of technology of chitosan fermentation by Aspergillus niger

通过单因素及正交实验对黑曲霉产壳聚糖的发酵工艺进行了优化。结果表明,最佳发酵条件为:70mL玉米浆,4%葡萄糖,1·2%硫酸镁,8·0×108孢子量,pH7·4～7·6,转速为130r/min,29℃发酵72h的壳聚糖的产率最高,达到18%。

The optimum condition for chitosan fermentation by Aspergillus niger were studied by single factor and orthogonal experiments.The optimal conditions of chitosan production were as follows：70mL/L corn steep liquor,4g/L glucose,1.2g/L of magnesium sulfate,8.0×108 spores,pH 7.47.6,130r/min,29℃ for 72h.There was a maximum productivity of chitosan 18%.