曲酸生产菌的^(60)Co-γ射线诱变选育及表征

Study on ^(60)Co-γ irradiation mutation breeding of kojic acid production strain and its characterization

为了获得高产曲酸菌株,采用不同剂量60Co-γ射线对出发菌株米曲霉进行辐射诱变处理。结果表明,当60Co-γ射线辐射剂量为800Gy时,对出发菌株的诱变效果最好,使突变菌株C8-128的曲酸产量由出发菌株的15·68g/L增加到61·5g/L,产量提高292·2%,经遗传稳定性实验,得出其有良好的遗传稳定性,产酸能力稳定。将筛选出的高产曲酸菌株C8-128通过菌落形态和摇瓶发酵培养特征与亲株进行比较,得出出发菌株经60Co-γ射线辐射诱变后产生的一些特征性变化。

Asperqillus oryzae were irradiated by different doses of 60Co-γ rays to obtain high-yielding strains of kojic acid.The results indicated that,when the dose of the 60Co-γ rays was 800Gy,it could have the best mutation effect to the start strain.The kojic acid yield of the mutant increased from original 15.68g/L to 61.5g/L,increased by 292.2%.It had a good genetic stability and stably acid producing.To obtain the characteristics changes of the C8-128 which has a high yielding of kojic acid,it was compared to the starting strain in colony morphology and fermentation characteristics in flask.