摘要
以废蚕丝的再生丝素液为原料,研究了有无超声作用、底物浓度、时间、温度、pH、加酶量等因素对碱性蛋白酶水解制备丝素肽的影响。结果表明,超声辅助酶解再生丝素液制备丝素肽最佳工艺为:底物浓度4·0%、酶浓度为4·0%、反应温度为45℃、反应时间为40min、pH为10·0、超声功率为200W。与水浴酶解相比,超声作用没有改变回收率与水解温度、pH、底物浓度、加酶量等因素的变化关系;但超声作用可提高回收率,缩短反应时间。
Taking regenerated solution of waste silk fibroin as material,the effects of the application of ultrasound,reactant concentration,time,temperature,pH and enzyme concentration on silk peptide hydrolyzed by alkaline protein enzyme and silk amino acid mixture were investigated by using single factor experiment.The optimal parameters were：reactant concentration 4.0%,enzyme concentration 4.0%,extraction temperature 45℃,reaction time 40min,pH 10.0,ultrasound power 200W.Compared with water bath hydrolyzing,ultrasound-assisted reaction is more effective with advantages in short reaction period and improving response rate.Though it cannot change the relationship among the recovery rate and reactant or enzyme concentration,temperature,pH,it can increase recovery rate and shorten time.
出处
《食品工业科技》
CAS
CSCD
北大核心
2010年第12期276-278,281,共4页
Science and Technology of Food Industry
关键词
废蚕丝素
超声
酶解
丝素肽
waste silk fibroin
ultrasound
enzyme hydrolysis
silk peptide
