毛细管电泳法测定增味剂中的5′-肌苷酸二钠和5′-鸟苷酸二钠的含量

Study on the determination of disodium 5'-inosinate,disodium 5'-guanylate by capillary electrophoresis

建立了同时测定5′-肌苷酸二钠和5′-鸟苷酸二钠的毛细管电泳法,研究了缓冲溶液种类、浓度、pH、电压等对测定的影响,对测定条件进行了优化。在波长250nm,分离电压15kV,5mmol·L-1磷酸二氢钠-5mmol·L-1硼酸-5%乙醇缓冲溶液(pH=10·3)中,增味剂中的5′-肌苷酸二钠和5′-鸟苷酸二钠在5min内得到了较好的分离,浓度与峰面积之间具有良好的线性关系。

This study established the method for directly determination and simultaneous separation of disodium 5＇-inosinate,disodium 5＇-guanylate using capillary electrophoresis.It also examined the effects of factors such as voltage,the type,concentration and acidity of the running buffer on determination.The best separation was achieved in the running buffer of 5mmol·L-1 NaH2PO4-5mmol·L-1 H3BO3（with 5% percent alcohol）at pH=10.3,and 15kV separation voltage,and with UV detection at 250nm.Disodium 5＇-inosinate and disodium 5＇-guanylate were completely separated and detected using the method within 5min.The concentration and peak area of disodium 5＇-inosinate and disodium 5＇-guanylate showed good linear relationships.