摘要
建立了双水解-柱前衍生高效液相色谱法测定鲍鱼中糖原含量的方法。以木瓜蛋白酶水解鲍鱼样品,释放出的糖原于2mol/L的三氟乙酸中,110℃条件下水解4h,彻底水解为葡萄糖,利用PMP柱前衍生高效液相色谱法对葡萄糖进行定量。该方法在葡萄糖含量为0·05～1·0mmol/L范围内线性关系良好(R2=0·9994),RSD为2·35%,平均回收率为96·53%,检测限为0·036mmol/L,定量限为0·06mmol/L。
The method of double hydrolysis PMP derivatization HPLC determining the glycogen content of abalone was established.Abalone samples were hydrolyzed by papain,to release glycogen,then the hydrolysate was further hydrolyzed for 4h by 2mol/L of trifluoroacetic acid at 110℃,to completely hydrolyze glycogen to glucose.The content of glucose was determined by utilizing PMP pre-column derivatization high-performance liquid chromatography.The linear relationship is good in range of 0.051.0mmol/L of the glucose content（R2=0.9994）.RSD was 2.35%,average recovery was 96.53%,the limit of detection was 0.036mmol/L,and the limit of quantification was 0.06mmol/L.
出处
《食品工业科技》
CAS
CSCD
北大核心
2010年第12期341-343,共3页
Science and Technology of Food Industry
基金
国家科技支撑计划(2008BAD94B05)
