SDG分离富集-1-(5-溴-2-吡啶偶氮)-2-萘酚-6-磺酸光度法测定环境样品中的微量汞

Spectrophotometric Determination of Mercury in Environmental Samples with 5-Br-PAN-S After Separation and Enrichment by Sulfhy dry lDextran Gel.

以５－Ｂｒ－ＰＡＮ－Ｓ为显色剂，研究了测定汞的显色条件。在ｐＨ９．０的氨性缓冲液中，形成１：１型的稳定配合物，配合物的最大吸收峰位于５５８ｎｍ波长处，表观摩尔吸光系数ε＝２．０×１０４Ｌ／（ｍｏｌ·ｃｍ）。汞量在０～８０μｇ／２５ｍＬ范围内符合比尔定律。采用巯基葡聚糖凝胶分离水中共存离子富集汞，提高了方法的选择性。本法用于水样分析。

Theconditionofcolourreactionofmercurywith1-(5-bromo-2-pyridylazo)-2-naphthol-6-sulphonicacid( 5-Br-PAN-S)arestudied.Mercuryformswith5-Br-PAN-Sa1:1stablecomplexinpH9.0.Themaximumab sorbanceofthecomplexisat558nmanditsapparentmolarabsorptivityis2.0× 104L/(mol·cm).Beer'slawisobeyedintherangeof0-80μ g/25mLformercury.Thecommonmetalionscanbeseparated,andtoenrichmercurybysulfhydrylcoton fiber,thus,greatlyimprovingtheselectivityandsensitivityofthesystem.Themethodhasbeenusedtod eterminethemercuryinenvironmentalsamplesandresultsaresatisfactory.