牡丹皮有效组分重组方对家兔血小板聚集及凝血功能的影响

Effects of Recombination of Effective Constituent of Cortex Moutan on Platelet Aggregation and Blood Coagulation in Rabbits

目的研究牡丹皮有效成分丹皮酚和芍药苷重组方对家兔血小板聚集和凝血功能的影响。方法采用比浊法以及凝血时间的检测方法对各项指标进行测定。结果丹皮酚和芍药苷配伍的高(丹皮酚50 mg.kg-1+芍药苷12.5 mg.kg-1)、中(丹皮酚25 mg.kg-1+芍药苷6.25 mg.kg-1)、低(丹皮酚12.5 mg.kg-1+芍药苷3.125 mg.kg-1)剂量组均能显著抑制胶原、二磷酸腺苷、花生四烯酸诱导的家兔血小板聚集;丹芍配伍高、中剂量组能明显延长血浆凝血酶时间及全凝血时间。结论牡丹皮有效成分丹皮酚和芍药苷重组方对家兔血小板聚集和凝血功能均有改善作用,为其临床应用提供了理论依据。

OBJECTIVE To study the effects of effective constituent of Cortex Moutan on platelet aggregation and blood coagulation in rabbits.METHODS Each index was measured by turbidimetric method and blood coagulation time.RESULTS High-dosage,middle-dosage and low-dosage of the administration of paeonol and paeoniflorin（paeonol 50 mg.kg？1＋paeoniflorin 12.5 mg.kg？1,paeonol 25 mg.kg？1＋paeoniflorin 6.25 mg.kg？1,paeonol 12.5 mg.kg？1＋paeoniflorin 3.125 mg.kg？1）,can significantly depress blood coagulation in rabbits derived by the collogen,adenosine diphosphate,arachidonic acid.High-dosage and middle-dosage of the administration of paeonol and paeoniflorin prominently prolong the plasma thrombin time and clotting time.CONCLUSION Effective constituent paeonol and paeoniflorin of Cortex Moutan can improve the platelet aggregation and blood coagulation in rabbits,provide the theory for clinical application.