灵丹草油提取工艺及其主要成分研究

Studies on the Optimum Extraction Technology for Oil and Its Main Ingredients of Laggera pterodonta(DC.) Benth

目的采用L9(34)正交试验法,研究灵丹草油最优提取工艺,分析灵丹草油的成分组成。方法以灵丹草油提取量为评价指标,以加水量、浸泡时间、提取时间为因素进行正交试验;采用GC-MS对灵丹草油主要成分进行分离分析。结果最佳提取工艺条件为加水10倍量,浸泡2h,加热提取8h;从灵丹草油中分离鉴定了20种主要化合物。结论优选的提取工艺稳定可行;α-可巴烯、β-芹子烯及1,2,3,4,4a,5,6,7-八氢-4a,8-二甲基-α-亚甲基-2-萘乙酸是灵丹草挥发油的主要成分,它们相对含量之和占到了灵丹草油的34.63%。

OBJECTIVE To optimize the extraction process of oil in Laggera pterodonta（DC.） Benth by orthogonal design L9（34）. And then analysis chemical constitutes in the Laggera pterodonta（DC.） Benth oil. METHODS The experiment was performed with the extraction amount of volatile oil as the index, and the volume of water, the time of immersion, the time of extraction as affecting factors. GC-MS method was used to analysis chemical constitutes in the Laggera pterodonta（DC.） Benth oil. RESULTS Optimized extraction process was as follows： the Laggera pterodonta（DC.） Benth was soaked in 10 times of water for 2 hours, then distilled for 8 hours. Twenty compounds were separated from the Laggera pterodonta（DC.） Benth oil and were identified. CONCLUSION The method is stable and practicable; α-copaene, β-selinene and 1,2,3,4,4a,5,6,7-octahydro- 4a,8-dimethyl-α-methylene-2-naphthaleneacetic acid were the main compounds of Laggera pterodonta （DC.） Benth oil, the amount of the three compounds can attain 34.63%.