HPLC同时测定乳癖消颗粒中三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1的含量

Determination of Notoginsenoside R1, Ginsenoside Rg1 and Ginsenoside Rb1 in Rupixiao Granule by HPLC

目的建立高效液相色谱法测定乳癖消颗粒中三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1含量。方法色谱柱为CAPCELL PAK C18（150 mm×4.6 mm,5μm）;流动相A为乙腈,B为水;流速：1.0 mL.min-1;检测波长为203 nm;洗脱程序：0~12 min,A相19%,B相81%;12~60 min,A为19%→36%,B为81%→64%。结果三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1保留时间分别为21,25,51 min,与各自相邻峰的分离度均在1.5以上。三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1的线性范围分别为0.100 2~2.00 4μg、0.418 8~8.376μg和0.187~3.74μg。三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1的回收率分别为98.5%,99.6%和96.5%,RSD分别为1.9%,1.3%和1.9%。结论本法简便、准确、重现性好,可用于乳癖消颗粒中三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1含量的同时测定。

OBJECTIVE To establish an HPLC method for determination of notoginsenoside R1,ginsenoside Rg1 and ginsenoside Rb1 in Rupixiao granule.METHODS The chromatographic conditions included C18 column（CAPCELL PAK,150 mm×4.6 mm,5 μm）,acetonitrile as mobile phase A,water as mobile phase B.The flow rate was 1.0 mL.min-1.The detection wavelength was 203 nm.Gradient elution： 0.12 min,A： 19%,B： 81%;12-60 min,A：19%→36%,B： 81→64%.RESULES The retention time of notoginsenoside R1,Ginsenoside Rg1 and Ginsenoside Rb1 was 21,25,51 min,respectively.The resolution was above 1.5.The linear range of notoginsenoside R1,ginsenoside Rg1 and ginsenoside Rb1 was 0.100 2-2.004 μg,0.418 8？8.376 μg and 0.187-3.74 μg.The average recovery was 98.5%,99.6% and 96.5%,RSD was 1.9%,1.3% and 1.9%,respectively.CONCLUSION The method is simple,accurate and reproduciable.It can be used for routine analysis of notoginsenoside R1,ginsenoside Rg1 and ginsenoside Rb1 in Rupixiao granule simultaneously.