摘要
目的在实践中应用并完善流感病毒分离培养的检验方法,获得流感毒株,为流感监测积累经验并奠定良好的基础。方法选择92份哨点医院送检标本进行流感荧光定量PCR检测,流感荧光定量PCR检测阳性的接种MDCK细胞,在MDCK细胞上培养和增殖流感病毒,根据细胞CPE程度确定收获病毒的时间,收获病毒后用血凝试验和血凝抑制试验进行鉴定。结果 92份标本中核酸检测阳性的28份,病毒培养阳性的12份,11份为B型毒株,1份为甲型流感毒株。结论取得良好的分离培养效果,该方法的掌握和应用为流感监测奠定了良好的基础。
Objective In practical application research and improvement of influenza virus inspection method for influenza monitoring influenza strains,and laid a good foundation.Methods Totally 92 point hospital specimens flu pandemic,fluorescence detection of quantitative fluorescence quantitative polymerase chain reaction（PCR） MDCK inoculation test positive for MDCK cells in the cell proliferation and cultivate flu virus,according to the degree of CPE determine harvesting virus,harvest time after the virus blood clots test and blood clots inhibit test appraisal.Results Among 92 specimens nucleic acid test positive for the 28,virus cultivation of 12 specemen,11 positive for B strain of influenza,1 for influenza A strains.Conclusion Good separation effect training,the method has laid a good foundation for influenza monitoring.
出处
《医药论坛杂志》
2010年第21期74-75,78,共3页
Journal of Medical Forum
