HPLC法同时测定刺五加果实中齐墩果酸和熊果酸的含量

Simultaneously Determination of Oleanolic Acid and Ursolic Acid in Medofenoxate Fruits by HPLC

目的建立HPLC同时测定刺五加果实中齐墩果酸和熊果酸含量的方法。方法采用Agilent XDB-C18色谱柱(4.6mm×250mm,5m),以甲醇-乙腈-0.3%磷酸溶液(30:60:10)为流动相,流速:0.8mL.min-1,柱温为室温,检测波长为210nm。结果齐墩果酸进样量在0.2052～3.2832μg范围内线性关系良好,r=0.9995,平均加样回收率为101.11%,RSD为1.69%(n=6);熊果酸进样量在0.2072～3.3152μg范围内线性关系良好,r=0.9993,平均加样回收率为98.51%,RSD为2.67%(n=6)。结论该方法灵敏快速、结果准确、重现性好,可用于刺五加果实中齐墩果酸和熊果酸含量的同时测定。

Objective To establish a HPLC method for the simultaneous determination of Oleanolic Acid and Ursolic Acid in Medofenoxate fruits.Methods Agilent XDB C18（4.6 mm×250 mm,5μm） column was applied,using methanol-acetonitrile-0.3% phosphoric acid（30 60 10） as mobile phase with the rate of 0.8mL·min-1.The column temperature was room temperature and the detective wavelength was 210nm.Results A good linearity was obtained over the range of 0.2052～3.2832μg for Oleanolic Acid（r=0.9995）.The average recovery（n=6） was 101.11%（RSD=1.69%）;A good linearity was obtained over the range of 0.2072～3.3152μg for Ursolic Acid（r=0.9993）.The average recovery（n=6） was 98.51%（RSD=2.67%）.Conclusion The method is practical,convenient,accurate,and can be used as the simulta-neous determination of Oleanolic Acid and Ursolic Acid in Medofenoxate fruits.