郁金香杂色综合症病原的免疫电镜鉴定与检测研究

Immuno gold identification and detection on pathogens of tulip breaking syndrome

免疫胶体金标记技术的鉴定结果表明，造成郁金香杂色综合症的主要病原是ＴＢＶ，其次也有少量的植株被ＴＢＢＶ、ＴＴＢＶ及ＣＭＶ所感染。ＴＢＶ及ＴＴＢＶ病毒主要在花瓣和叶片中增殖与积累，并通过种球传给后代。ＴＢＶ和ＴＢＢＶ可共同识别某些植株的病原，说明二者应属同一种病毒的不同株系。

Identification of pathogens of tulip breaking syndrome was conducted with immunogold labelling method.The results showed that the most of the plants with the symptom were caused by the infection of TBV;TBBV,TTBV and CMV were detected occasionally.TBV multipled and accumulated mainly in the petal and leaves of tulip,and were then transmitted to the progeny through the bulbs.Some pathogens could be recognized by both TBV and TBBV antisera,which suggested that they should belong to different isolines of the virus TBV.