近红外光谱法快速定量分析那格列奈

Rapid Quantitative Analysis of Nateglinide Based on Near Infrared Spectroscopy

采用近红外漫反射光谱分析技术和化学计量学的方法对那格列奈进行快速无损的定量分析.采集41份不同浓度系列的那格列奈样品测量近红外漫反射光谱,用多元散射校正结合一阶导数的光谱预处理方法,结合偏最小二乘法(PLS)建立那格列奈的定量分析模型.结果表明:交叉验证均方根误差(RMSECV)为0.448 5,决定系数R2为0.999 8;预测均方根误差(RMSEP)为0.196 0.方法精密度RSD为1.1%(n=6),加标回收率为95.55%.结果表明,用近红外光谱分析技术对那格列奈进行定量分析结果准确可靠,方法简便快速,可推广用于此类样品的工业在线分析.

Near infrared spectroscopy（NIRS） and chemometrics methods were applied in fast,non-destructive,quantitative analysis of nateglinide.The quantitative analysis model was established by 41 different series near-infrared reflectance spectroscopy of nateglinide samples,combined with multiplicative scatter correction,first derivative and partial least squares（PLS）.The cross-validation root mean square error（RMSECV） was 0.448 5 and determination coefficient R2 was 0.999 8;root mean square error of prediction（RMSEP） was 0.196 0. Precision of the method RSD was 1.1%（n=6）,the average recovery rate was 95.55%.It is shown that near-infrared spectroscopy technology is accurate and reliable for nateglinide quantitative analysis.The method is simple and rapid,and it may be extended to industrial on-line analysis of such samples.