摘要
研究了对苯醌活化法在纤维素滤纸膜载体上固定化猪胰脂肪酶的最佳条件。结果表明，当对苯醌浓度为０．０１ｇ／ｍｌ，活化６０ｍｉｎ，与浓度为０．００５ｇ／ｍｌ的酶的ｐＨ８．０的磷酸盐缓冲溶液于４℃交联２４ｈ，获得的固定化酶活最高，为０．４４Ｕ／ｃｍ２。固定化酶最适温度３５℃，最适ｐＨ９．０。
The conditions of crosslinking porcine pancreas lipase to cellulose membrane carrier by means of benzoquinone were studied.The results showed that the maximum 0.44U/cm 2activity of the immobilized lipase was given when the benzoquinone content was 0.01g/ml,activation time was 60min,lipase content in pH 8.0 phosphate buffer was 0.005g/ml and crosslinking reaction time was 24h at 4℃.The optimum operating temperature and pH of the immobilized lipase was 35℃ and 9.0 respectively.
出处
《中国粮油学报》
EI
CAS
CSCD
北大核心
1999年第2期44-47,共4页
Journal of the Chinese Cereals and Oils Association
基金
国家自然科学基金
