脐带血有核细胞移植治疗肌萎缩侧索硬化的临床研究

Clinical study of treating amyotrophic lateral sclerosis with transplantation of umbilical cord blood nuclear cells

目的观察脐带血有核细胞治疗肌萎缩侧索硬化(ALS)的疗效并探讨机制。方法采集新生儿脐带血,获取含干细胞的有核细胞混悬液,每份平均含有核细胞4.29×108个,通过腰椎穿刺,将其移植入蛛网膜下腔。结果移植后第5天患者单手握力较移植前平均增加1.68 kg;移植后第1个月94.46%患者病情好转;第2个月5.12%持续好转,73.04%保持稳定;第3个月36.06%保持稳定;第4个月18.85%保持稳定;第5个月10.61%保持稳定。结论脐带血有核细胞蛛网膜下腔移植在一定时间内可有效缓解ALS的临床症状。

Objective To investigate the curative effect of umbilical cord blood nuclear cells transplantation on treating ALS and the mechanism of this therapy.Methods Umbilical cord blood was collected,and the nuclear cell suspensions containing stem cells were made and injected into subarachnoid space of the patients.Each suspension contained 4.29×108 nuclear cells averagely.The movement functions of the cases were evaluated.Results 5 days after transplantation,the single hand holding strength of patients averagely increased 1.68 kg compared with before treatment.94.46% patients improved in the first month.5.12% patients continued to improve and 73.04% patients maintain in stable condition in the second month.36.06% patients maintained in stable condition in the third month.18.85% patients maintained in stable condition in the forth month.10.61% patients maintained in stable condition in the fifth month.Conclusion Umbilical cord blood nuclear cells transplantation may improve patient＇s movement function in a certain time.