阿魏酸钠对大鼠缺血心肌tTG表达的影响

Effects of sodium ferulate on tTG expression in rat ischemic myocardium

目的:探讨组织型转谷氨酰胺酶(tTG)在大鼠缺血心肌的表达及阿魏酸钠(SF)对其表达治疗的影响。方法:60只大鼠随机分为12组,每组5只:正常对照组;模型组包括注射盐酸异丙肾上腺素(Iso)2h、4h、6h、12h、24h、48h、72h、1周及3周组;贝那普利(Bena)阳性对照组;SF治疗组。除正常对照组外,其他各组大鼠均皮下注射Iso(15mg·kg-1);阳性对照组同步注射贝那普利;SF治疗组同步注射SF。并于给药后对应时间取大鼠心脏,采用免疫组织化学法及RT-PCR方法检测各组大鼠心肌组织中tTG的表达。结果:注射Iso2h后,大鼠心肌组织中tTG表达较正常对照组增加,但差异无显著性(P>0.05);随着注射Iso时间的延长,3周达到高峰,与正常对照组比较差异有显著性(P<0.01)。tTGmRNA表达量在24h达到高峰,与正常对照组比较差异有显著性(P<0.01);自48h后其表达逐渐下降,但与正常对照组比较差异仍有显著性(P<0.05)。SF治疗组tTG的表达量降低,其基因表达亦明显减少,与Iso组比较差异有显著性(P<0.05或P<0.01)。结论:在大鼠心肌缺血模型中,心肌组织tTG表达增加,加速了心肌缺血的进程;SF抑制tTG的表达,可降低心肌缺血的进程。

Objective To investigate the expression of tissue transglutaminases（tTG）in rat ischemic myocardium and the role of sodium ferulate（SF）in rat myocardial ischemia.Methods 70male Wistar rats were randomly divided into fourteen group （n=5）,control groups,model groups （2h,4h,6h,12h,24h,48h,72h, 1week and 3weeks after injection of isoproterenol hydrochloride（Iso）） （the control group and 3weeks group were divided into two groups）,positive control group and SF group.Except control group,all the rats in the other groups were treated with Iso （15 mg·kg-1）.In addition,the rats in positive control group were injected synchronously with bean and the rats in SF group were injected synchronously with SF.The tTG expression was observed before and after SF treatment by immunohistochemistry and RT-PCR.Results The tTG protein expression in rat ischemic myocardium 2hafter Iso injection was gradually increased compared with control group （P0.05）.With the prolongation of time of Iso injection,the tTG protein expression arrived a peak at 3week compared with control group （P0.01）.The tTG mRNA expression at 24hreached peak compared with control group and was gradually decreased at 48h （P0.05or P0.01）.The tTG protein expression after SF intervention was gradually decreased compared with model group （P0.01）.The tTG mRNA expressionwasalsogradually decreased compared with model group （P0.05or P0.01）.Conclusion The increased tTG expression accelerate the process of myocardial ischemia in rat myocardial ischemic model.SF can inhibit the tTG expression and reduce the process of myocardial ischemia.