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移植共培养的骨髓间充质干细胞与胰岛细胞对糖尿病大鼠的降血糖作用 被引量:2

Effects of bone marrow mesenchymal stem cells cocultured with pancreatic cells on hyperglycemia in streptozotocin-induced diabetic rats
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摘要 目的:观察与胰岛细胞共培养的大鼠骨髓间充质干细胞(BMSCs)能否转化为胰岛样细胞,并探讨将其移植入糖尿病大鼠体内对血糖的控制效果。方法:体外共培养BMSCs及胰岛细胞;18只雄性SD大鼠通过腹腔注射链脲佐菌素(STZ)制备糖尿病大鼠模型,分为PBS组、BMSCs组、BMSCs与胰岛细胞共培养组。将Brdu标记的细胞从尾静脉移植入糖尿病大鼠模型体内,观察临床疗效,免疫组织化学染色确定Brdu标记的细胞及胰岛素抗体在胰腺的分布。结果:细胞移植后用血糖仪检测大鼠尾静脉血糖,PBS组血糖较移植前无明显下降(P>0.05),BMSCs组及共培养组大鼠血糖移植后14d血糖开始下降,与移植前比较差异有显著性(P<0.01),共培养组大鼠移植后血糖较BMSCs组血糖明显下降,组间比较差异有显著性(P<0.01)。免疫组织化学方法检测,BMSCs组及共培养组Brdu标记的细胞在细胞核显色,为棕黑色。其相应的胰岛素表达阳性,主要显色在胞浆,为棕红色。PBS组大鼠胰腺未发现Brdu标记细胞及胰岛素表达阳性。结论:大鼠BMSCs在体内微环境下经尾静脉移植入糖尿病大鼠体内,可以降低大鼠血糖,而经体外微环境与胰岛细胞共培养的BMSCs移植入糖尿病大鼠体内后能起到相同作用,且降糖作用强于前者。 Objective To observe if the bone marrow mesenchymal stem cells(BMSCs)cocultured with pancreatic cells can be transformed into islet-like cells,and clarify the effect of blood glucose control of BMSCs in diabetic rats.Methods BMSCs and pancreatic cells were concultured in vitro.18SD rats were introperitonally injected with streptozotocin (STZ)to set up animal models of diabetes and the rat models were randomly divided into PBS group,BMSCs group and BMSCs cocultured with pancreatic cells group (n=6).The bromodeoxyuridine (Brdu)-labeled cells were transplanted into STZ-inducd diabetic rats by caudal vein,the clinical efficacy was observed.The distrubition of Brdu-labeled cells in the pancreas was examined by immunohistochemistry. Results The blood glucose in caudal vein was detected by blood glucose detection instrument.The blood glucose level in PBS group didn’t change compared with before transplantation (P0.05).The blood glucose level was reduced from the 14th day after transplantation in BMSCs group and BMSCs cocultured with pancreatic cells group, there were significant differences compared with before transplantation(P0.01).There was significantdifferenceof the blood glucose level after transplantation between BMSCs group and BMSCs cocultured with pancreatic cells group (P0.01).The immunohistochemical results showed that the Brdu-labeled cells with positive insulin-expression were tested in the BMSCs group and BMSCs cocultured with pancreatic cells group,but they were not found in PBS group.Conclusion The cultured cells could reduce hyperglycemia in the STZ-induced diabetic rats by caudal vein.BMSCs could also reduce hyperglycemia in the STZ-induced diabetic rats by caudal vein.The effect of the later was better than the former.
出处 《吉林大学学报(医学版)》 CAS CSCD 北大核心 2010年第6期1079-1083,1207,共6页 Journal of Jilin University:Medicine Edition
基金 河北省卫生厅重大科技攻关项目资助课题(07147)
关键词 骨髓间充质干细胞 胰岛细胞 共培养 糖尿病 实验性 移植 bone marrow mesenchymal stem cells pancreatic cells coculture diabetes experimental transplantation
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