TTC法测定4种针茅种子生活力染色技术的研究

Study on dying technique of TTC method for testing seed viability of four Stipa

采用TTC法测定4种针茅种子生活力,结果表明TTC法能够较好的反映4种针茅种子的生活力。试验针对影响TTC染色效果的5个因子进行了全面的梯度试验与数学分析,即浸种温度、浸种时间、TTC浓度、染色温度和染色时间,其中浸种温度、浸种时间和TTC浓度对染色效果起主要作用,并得到了测定4种针茅种子最佳染色技术条件,分别为(浸种温度-浸种时间-TTC浓度-染色温度-染色时间):短花针茅种子30℃-6 h-0.3%-40℃-3 h;大针茅种子30℃-3 h-0.1%-40℃-6 h;克氏针茅种子30℃-6 h-0.3%-35℃-18 h;贝加尔针茅种子35℃-12 h-0.3%-30℃-18 h。

The seed viability of 4 Stipa was studied with TTC method and the results indicated that it was a proper method for the seed viability test.The effects of 5 experiment factors,including soaking time,soaking temperature,TTC concentration,dying temperature and dying time were studied as well.The main factors affecting the dying effect were soaking time,soaking temperature and TTC concentration mainly.The optimum dying conditions（soaking time, soaking temperature,TTC concentration,dying temperature,dying time ）for Stipa breviflora,S. grandis,S.krylovii and S. baicalensis were 30 ℃,6h,0.3%,40 ℃,3h;30 ℃, 3h,0.1%,40 ℃, 6 h;3℃,6 h,0.3%,35 ℃,18 h and 35 ℃,12 h,0.3%,30 ℃,18 h respectively.